Rapid identification and cloning of bacterial transferrin and lactoferrin receptor protein genes.
نویسندگان
چکیده
The sequences of genes encoding the transferrin and lactoferrin receptor proteins from several bacterial species were analyzed for areas of identity in the predicted protein sequences. Degenerate oligonucleotide primers were designed and tested for their ability to amplify portions of the receptor genes. Primer pairs capable of amplifying products of the tbpA/lbpA or tbpB/lbpB genes from all species possessing these receptors were identified.
منابع مشابه
Cloning and expression of the Moraxella catarrhalis lactoferrin receptor genes.
The lactoferrin receptor genes from two strains of Moraxella catarrhalis have been cloned and sequenced. The lfr genes are arranged as lbpB followed by lbpA, a gene arrangement found in lactoferrin and transferrin receptor operons from several bacterial species. In addition, a third open reading frame, orf3, is located one nucleotide downstream of lbpA. The deduced lactoferrin binding protein A...
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عنوان ژورنال:
- Journal of bacteriology
دوره 178 24 شماره
صفحات -
تاریخ انتشار 1996